Expression of a metallothionein A1 gene of Pisum sativum in white poplar enhances tolerance and accumulation of zinc and copper.
Identifieur interne : 002A90 ( Main/Exploration ); précédent : 002A89; suivant : 002A91Expression of a metallothionein A1 gene of Pisum sativum in white poplar enhances tolerance and accumulation of zinc and copper.
Auteurs : Adelaide Turchi [Italie] ; Ivano Tamantini ; Alessandro M. Camussi ; Milvia Luisa RacchiSource :
- Plant science : an international journal of experimental plant biology [ 1873-2259 ] ; 2012.
Descripteurs français
- KwdFr :
- Adaptation physiologique (MeSH), Antioxydants (métabolisme), Cuivre (métabolisme), Espèces réactives de l'oxygène (métabolisme), Métallothionéine (génétique), Métallothionéine (métabolisme), Pois (génétique), Populus (génétique), Populus (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Végétaux génétiquement modifiés (métabolisme), Zinc (métabolisme).
- MESH :
- génétique : Métallothionéine, Pois, Populus, Protéines végétales.
- métabolisme : Antioxydants, Cuivre, Espèces réactives de l'oxygène, Métallothionéine, Populus, Protéines végétales, Végétaux génétiquement modifiés, Zinc.
- Adaptation physiologique.
English descriptors
- KwdEn :
- Adaptation, Physiological (MeSH), Antioxidants (metabolism), Copper (metabolism), Metallothionein (genetics), Metallothionein (metabolism), Peas (genetics), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (metabolism), Populus (genetics), Populus (metabolism), Reactive Oxygen Species (metabolism), Zinc (metabolism).
- MESH :
- chemical , genetics : Metallothionein, Plant Proteins.
- chemical , metabolism : Antioxidants, Copper, Metallothionein, Plant Proteins, Reactive Oxygen Species, Zinc.
- genetics : Peas, Populus.
- metabolism : Plants, Genetically Modified, Populus.
- Adaptation, Physiological.
Abstract
Metallothioneins (MT) play an important role in heavy metal detoxification and homeostasis of intracellular metal ions in plant. In this study, two transgenic lines expressing MT type 2 gene (PsMT(A1)) from Pisum sativum, a regenerated non transformed line NT and clone AL22, selected as heavy metal tolerant, were characterized in presence of the heavy metals for the ability to accumulate zinc and copper and to activate antioxidative enzyme defences: superoxide dismutase, catalase, ascorbate peroxidase. The levels of expression of MT type 2 gene assessed by RT-qPCR confirmed the gene over-expression in transgenic lines and evidenced in NT and AL22 the up-regulation of gene transcription by zinc and copper. Transgenic poplar lines during heavy metal stress displayed increased ability to translocate and accumulate zinc and copper compared with NT and AL22. The antioxidant enzyme defence was differently activated in response to metals in the transgenic lines without a significant increase of ROS. These results suggested that PsMT(A1) could play a role in ROS scavenging leading to enhanced metal tolerance and increased zinc and copper sequestration in root and leaf.
DOI: 10.1016/j.plantsci.2011.11.008
PubMed: 22195577
Affiliations:
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Camussi</name></author><author><name sortKey="Racchi, Milvia Luisa" sort="Racchi, Milvia Luisa" uniqKey="Racchi M" first="Milvia Luisa" last="Racchi">Milvia Luisa Racchi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22195577</idno><idno type="pmid">22195577</idno><idno type="doi">10.1016/j.plantsci.2011.11.008</idno><idno type="wicri:Area/Main/Corpus">002B95</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002B95</idno><idno type="wicri:Area/Main/Curation">002B95</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002B95</idno><idno type="wicri:Area/Main/Exploration">002B95</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Expression of a metallothionein A1 gene of Pisum sativum in white poplar enhances tolerance and accumulation of zinc and copper.</title><author><name sortKey="Turchi, Adelaide" sort="Turchi, Adelaide" uniqKey="Turchi A" first="Adelaide" last="Turchi">Adelaide Turchi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Agricultural Biotechnology, Genetics section, via Maragliano 77, 50144 Florence, Italy.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Agricultural Biotechnology, Genetics section, via Maragliano 77, 50144 Florence</wicri:regionArea><wicri:noRegion>50144 Florence</wicri:noRegion></affiliation></author><author><name sortKey="Tamantini, Ivano" sort="Tamantini, Ivano" uniqKey="Tamantini I" first="Ivano" last="Tamantini">Ivano Tamantini</name></author><author><name sortKey="Camussi, Alessandro M" sort="Camussi, Alessandro M" uniqKey="Camussi A" first="Alessandro M" last="Camussi">Alessandro M. Camussi</name></author><author><name sortKey="Racchi, Milvia Luisa" sort="Racchi, Milvia Luisa" uniqKey="Racchi M" first="Milvia Luisa" last="Racchi">Milvia Luisa Racchi</name></author></analytic><series><title level="j">Plant science : an international journal of experimental plant biology</title><idno type="eISSN">1873-2259</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptation, Physiological (MeSH)</term><term>Antioxidants (metabolism)</term><term>Copper (metabolism)</term><term>Metallothionein (genetics)</term><term>Metallothionein (metabolism)</term><term>Peas (genetics)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Reactive Oxygen Species (metabolism)</term><term>Zinc (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adaptation physiologique (MeSH)</term><term>Antioxydants (métabolisme)</term><term>Cuivre (métabolisme)</term><term>Espèces réactives de l'oxygène (métabolisme)</term><term>Métallothionéine (génétique)</term><term>Métallothionéine (métabolisme)</term><term>Pois (génétique)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Végétaux génétiquement modifiés (métabolisme)</term><term>Zinc (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Metallothionein</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antioxidants</term><term>Copper</term><term>Metallothionein</term><term>Plant Proteins</term><term>Reactive Oxygen Species</term><term>Zinc</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Peas</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Métallothionéine</term><term>Pois</term><term>Populus</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antioxydants</term><term>Cuivre</term><term>Espèces réactives de l'oxygène</term><term>Métallothionéine</term><term>Populus</term><term>Protéines végétales</term><term>Végétaux génétiquement modifiés</term><term>Zinc</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adaptation, Physiological</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adaptation physiologique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Metallothioneins (MT) play an important role in heavy metal detoxification and homeostasis of intracellular metal ions in plant. In this study, two transgenic lines expressing MT type 2 gene (PsMT(A1)) from Pisum sativum, a regenerated non transformed line NT and clone AL22, selected as heavy metal tolerant, were characterized in presence of the heavy metals for the ability to accumulate zinc and copper and to activate antioxidative enzyme defences: superoxide dismutase, catalase, ascorbate peroxidase. The levels of expression of MT type 2 gene assessed by RT-qPCR confirmed the gene over-expression in transgenic lines and evidenced in NT and AL22 the up-regulation of gene transcription by zinc and copper. Transgenic poplar lines during heavy metal stress displayed increased ability to translocate and accumulate zinc and copper compared with NT and AL22. The antioxidant enzyme defence was differently activated in response to metals in the transgenic lines without a significant increase of ROS. These results suggested that PsMT(A1) could play a role in ROS scavenging leading to enhanced metal tolerance and increased zinc and copper sequestration in root and leaf.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22195577</PMID><DateCompleted><Year>2012</Year><Month>09</Month><Day>27</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2259</ISSN><JournalIssue CitedMedium="Internet"><Volume>183</Volume><PubDate><Year>2012</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Plant science : an international journal of experimental plant biology</Title><ISOAbbreviation>Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Expression of a metallothionein A1 gene of Pisum sativum in white poplar enhances tolerance and accumulation of zinc and copper.</ArticleTitle><Pagination><MedlinePgn>50-6</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.plantsci.2011.11.008</ELocationID><Abstract><AbstractText>Metallothioneins (MT) play an important role in heavy metal detoxification and homeostasis of intracellular metal ions in plant. In this study, two transgenic lines expressing MT type 2 gene (PsMT(A1)) from Pisum sativum, a regenerated non transformed line NT and clone AL22, selected as heavy metal tolerant, were characterized in presence of the heavy metals for the ability to accumulate zinc and copper and to activate antioxidative enzyme defences: superoxide dismutase, catalase, ascorbate peroxidase. The levels of expression of MT type 2 gene assessed by RT-qPCR confirmed the gene over-expression in transgenic lines and evidenced in NT and AL22 the up-regulation of gene transcription by zinc and copper. Transgenic poplar lines during heavy metal stress displayed increased ability to translocate and accumulate zinc and copper compared with NT and AL22. The antioxidant enzyme defence was differently activated in response to metals in the transgenic lines without a significant increase of ROS. These results suggested that PsMT(A1) could play a role in ROS scavenging leading to enhanced metal tolerance and increased zinc and copper sequestration in root and leaf.</AbstractText><CopyrightInformation>Copyright © 2011 Elsevier Ireland Ltd. 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Camussi</name><name sortKey="Racchi, Milvia Luisa" sort="Racchi, Milvia Luisa" uniqKey="Racchi M" first="Milvia Luisa" last="Racchi">Milvia Luisa Racchi</name><name sortKey="Tamantini, Ivano" sort="Tamantini, Ivano" uniqKey="Tamantini I" first="Ivano" last="Tamantini">Ivano Tamantini</name></noCountry><country name="Italie"><noRegion><name sortKey="Turchi, Adelaide" sort="Turchi, Adelaide" uniqKey="Turchi A" first="Adelaide" last="Turchi">Adelaide Turchi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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